The southbound Royal Parade service lane near Grattan Street will be closed for periods of this weekend and next weekend.
The Metro Tunnel Project will be investigating services under the road in the vicinity of the intersection.
Bike riders and traffic will be diverted around the work and into the southbound centre lane.
Signage and traffic controllers will be in place while works take place.
Pedestrian access will be maintained with minor localised pedestrian diversions in place.
Access to University of Melbourne via Gate 11 will be maintained under traffic control.
Royal Parade will be returned to current arrangements when works are not occurring.
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