Riders should be on the look out for disruption on the Rathdowne Street route in North Carlton from next week.
City West Water is renewing a section of water main that dates to 1888.
The project, between Curtain Street and Macpherson Street, is expected to take two months.
Traffic management will be in place to ensure bike riders, motorists and pedestrians are able to move through the project area safely and efficiently.
This will include merging the bike lane with the single traffic lane. Speeds will be reduced during the works.
The project will also include renewing water service connections to some properties.