The shared path through Thomas Kidney Reserve will be closed to users from next Monday 16 March for up to four weeks.
This will close the busy path that connects the Capital City Trail with the Merri Creek Trail via the substation at the Russell Community Garden.
Diversions will be in place directing bike riders onto Holden Street and Bennett Street.
The City of Yarra is replacing the main shared path in the Reserve with a concrete path, three metres wide.
Other existing asphalt paths are also being replaced.
Sections of the walking trails will also be closed and detours in place.