It depends if you've got a "low" or "flat" spot, or a "high" spot.
It's not unheard of for tyres to reseat slightly off after fixing a puncture (most commonly it's a bead not popped up out of the central channel & into the rim bead properly, creating a low spot). Deflate the tyre to about 20-30 PSI, so it's soft enough to squeeze around on the rim. There is a moulded line all the way around the tyre on both sides, about 8mm from the bead edge; this line should be evenly visible just clear of the rim edge when the tyre is seated properly. If this line dips below the lip of the rim, there is your flat spot. There are a few steps of persuasion to pop a bead in this instance. The first is while the tyre is soft, use the balls of your hands to push the tyre up on the rim... Rest the wheel against your hips, flat spot upwards and on the opposite side of the wheel to your body. Wrap your fingers over the tyre & push on the sidewall with the balls of your hands, working from the ends of the flat spot towards the centre. Gradually increasing the tyre pressure between pushes will assist the tyre up.
If it's a really reluctant one (often caused by rubber rim tapes in single wall rims sitting up on the side of the central channel), fully deflate the tyre and smear some liquid detergent on the inside of the rim in the troubled area. (Use detergent as a lube here, because it's water soluble and can easily be washed off when you're done, so you won't get oil in your brakes
) Repeat the above process. The detergent will help the tyre slide up the rim & into place. Just make sure you're not blowing the tyre off the rim in another area, otherwise you could get a bit of a shock.....
In really tough cases, you can pump the tyre up a lot higher than the recommended maximum pressure, but do so gradually, ensuring that other sections are not blowing off. Aas soon as the bead has popped into place, reduce the pressure to your normal setting.
If you have a high spot, again reduce pressure to about 30 PSI and press the tyre down into the rim at that point. When you're happy that it's evenly seated, inflate, continually checking that it's remaining seated. If the tyre continues to lift at a certain place, fully deflate and inspect the bead for wear. If the lip is flattened or detaching so it no longer seats properly in the rim bead, then the tyre must be replaced; there is no way to repair it.