Police say the 30-year-old woman was threatened, pulled into bushes near a bike path and assaulted around 8pm on Wednesday.
The woman was earlier speaking with the man on a tram travelling from Box Hill to Port Melbourne. They got off the tram near the Graham Street overpass and walked along a bike path together, before the man threatened the woman.
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Did you see something? Call Crime Stoppers on 1800 333 000.